Hingga saat ini Institut Studi Islam Darussalam telah melakukan kerjasama akademik dengan beberapa perguruan tinggi dalam negeri mapun luar negeri, dan saat ini telah tercatat 32 Perguruan Tinggi yang telah bekersama sama diantaranya:

1. International Islamic University, Malaysia. 11 November 2000
2. Institute of Leadership and Management, Lahore, Pakistan. 10 Mei 2002
3. University of Malaya, Malaysia. 2005
4. Universitas Islam Indonesia (UII), Yogyakarta. 20 Mei 2006
5. Universitas Negeri Makasar, Makasar. 2006
6. Universitas Brawijaya (UNIBRAW), Malang. September 2006
7. African International University, Khourtoum, Sudan. 2 Juli 2007
8. International Islamic University, Islamabad, Pakistan. 2 Juli 2007
9. Institute of Asian Studies, Zaqaziq University, Egypt. 2 Juli, 2007
10. Mu’assasah Darul Hadits al-Hasaniyyah, Rabat Morocco. 28 Juli 2008.
11. Majma’ Syekh Ahmad Kaftharu Damascus, Syiria. 20 April 2008.
12. Al-fathul Islamy University, Damascus, Syiria. 20 April 2008
13. National University, Malaysia. 1 September 2008
14. Institute of Education, Tuanku Bainun, Pinang, Malaysia. 24 November 2008
15. Ibnu Sina Institute of Humanities, Paris. 11 Pebruari  2009.
16. Sana University, Sana, Yemen. 11 Pebruari 2009.
17. Islamic University of Europe, Rotterdam, Holland, 12 Pebruari 2009
18. Iman University, Sana, Yemen. 14 Pebruari 2009
19. Suez Canal University, Suez, Egypt. 16 Pebruari 2009
20. Universitas Gadjah Mada (UGM), Yogyakarta. 1 Maret 2009.
21. Universitas Islam Antarbangsa Selangor Malaysia. 26 Mei 2009.
22. Academic of Islamic Banking Kazakhstan. 26 Mei 2009.
23. Islamic University of Europe Istanbul Turkey. 26 Mei 2009.
24. Quad-l-Azzam University (QAU), Islambad Pakistan. 05 Desember 2009.
25. Ali Bait University, Yordan. 25 November 2009.
26. Damascus University, Damascus, Syiria. 26 November 2009.
27. Fateh University, Istanbul Turkey. 03 Desember 2009.
28. Aligarh Muslim University, Aligarh India. 15 Desember 2009.
29. The Istanbul Foundation for Science and Culture. 03 Desember 2009.
30. University of the Punjab, Lahore Pakistan. 07 Desember 2009.
31. Markfield Institute of Higher Education, Leicester, United Kingdom, 15 April  2010.
32. Universitas Negeri Yogyakarta (UNY), Yogyakarta Indonesia. 19 Mei 2010.